User:Paul Rothenberg/Notebook/Pauls Notebook/2013/06/24: Difference between revisions
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== | ==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb Citrate, Tris, Phosphate, and Acetate Catalysts in Toluene Suspension== | ||
Today, Dhea and I are going to start running the reaction in Toluene. Some observations from last week were to make the reference solution first, so more of the Mb is concentrated in the reaction. The reaction will also be centrifuged, because we were running it like so and got results (and we also were running low on syringe filters). We are not mixing up a solution of H<sub>2</sub>O<sub>2</sub> in Toluene; instead, we are keeping them separate and adding them each into the reaction to prevent the two from separating. That happened on Friday, and we calculated how much of each should be added to compensate. | |||
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Reaction of OPD+H2O2 with Mb Citrate, Tris, Phosphate, and Acetate Catalysts in Toluene SuspensionToday, Dhea and I are going to start running the reaction in Toluene. Some observations from last week were to make the reference solution first, so more of the Mb is concentrated in the reaction. The reaction will also be centrifuged, because we were running it like so and got results (and we also were running low on syringe filters). We are not mixing up a solution of H2O2 in Toluene; instead, we are keeping them separate and adding them each into the reaction to prevent the two from separating. That happened on Friday, and we calculated how much of each should be added to compensate.
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