User:Paul Rothenberg/Notebook/Pauls Notebook/2013/06/04: Difference between revisions
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Going to run the same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/31 Friday] and [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/06/03 yesterday], but with Mb/Tris. | Going to run the same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/31 Friday] and [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/06/03 yesterday], but with Mb/Tris. | ||
Based on the inconsistencies of the previous reactions, I am going to first take the reference spectrum of OPD and Mb in Isopropanol AFTER centrifuging it. Then, I am going to run the reaction in 20 minute intervals and use fresh pipette tips each time to see if that makes a difference. Also, I'm going to try to use more than 1 mL aliquots of solution to centrifuge, so I can hopefully avoid pulling any of the collected precipitate into the cuvette. I am also going to only remove the sample to be centrifuged right as it needs to be; not isolated from the original reaction as I had done previously. | Based on the inconsistencies of the previous reactions, I am going to first take the reference spectrum of OPD and Mb in Isopropanol AFTER centrifuging it. Then, I am going to run the reaction in 20 minute intervals and use fresh pipette tips each time to see if that makes a difference. Also, I'm going to try to use more than 1 mL aliquots of solution to centrifuge, so I can hopefully avoid pulling any of the collected precipitate into the cuvette. I am also going to only remove the sample to be centrifuged right as it needs to be; not isolated from the original reaction as I had done previously. | ||
For the reference solution, I am going to use 6.74 mL of OPD and 0.75 mL of Mb solutions, and what I would have added in as H<sub>2</sub>O<sub>2</sub> will be Isopropanol. Hopefully that will give a better reference spectrum. | |||
Revision as of 06:03, 4 June 2013
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Reaction of OPD+H2O2 with Mb/Tris Catalyst in Isopropanol SuspensionGoing to run the same procedure as Friday and yesterday, but with Mb/Tris. Based on the inconsistencies of the previous reactions, I am going to first take the reference spectrum of OPD and Mb in Isopropanol AFTER centrifuging it. Then, I am going to run the reaction in 20 minute intervals and use fresh pipette tips each time to see if that makes a difference. Also, I'm going to try to use more than 1 mL aliquots of solution to centrifuge, so I can hopefully avoid pulling any of the collected precipitate into the cuvette. I am also going to only remove the sample to be centrifuged right as it needs to be; not isolated from the original reaction as I had done previously. For the reference solution, I am going to use 6.74 mL of OPD and 0.75 mL of Mb solutions, and what I would have added in as H2O2 will be Isopropanol. Hopefully that will give a better reference spectrum.
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