User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/30: Difference between revisions

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*<b>Data</b>:
*<b>Data</b>:


The protein did not fully dissolve in solution, similar to yesterday. However, the reaction still proceeded forwards. Some temperatures favored the reaction more than others, seen in the graphs below. The 25°C and 45°C reactions had  particularly large absorbance spectrum at 460 nm, compared to the rest of the reactions. The 5°C, 15°C, and 35°C reactions did not have smooth curves; instead, had multiple peaks and relatively low absorbances, if any, at 460 nm.  
The protein did not fully dissolve in solution, similar to yesterday. However, the reaction still proceeded forwards. Some temperatures favored the reaction more than others, seen in the graphs below. The 25°C and 45°C reactions had  particularly large absorbance spectrum at 420 nm, compared to the rest of the reactions. The 5°C, 15°C, and 35°C reactions did not have smooth curves; instead, had multiple peaks and relatively low absorbances, if any, at 420 nm.  


[[Image:Mb_Acetate_H2O_5C_WORKUP_GRAPH.png]]
[[Image:Mb_Acetate_H2O_5C_WORKUP_GRAPH.png]]

Revision as of 08:05, 30 May 2013

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30 May 2013

  • Objectives:
  • 1. Continue analysis of reaction of OPD+H2O2 in Mb Catalyst in aqueous solution
  • Procedure:
  • 1. Follow same procedure as 5/20 with Mb in Acetate buffer with H2O as the solvent
  • Data:

The protein did not fully dissolve in solution, similar to yesterday. However, the reaction still proceeded forwards. Some temperatures favored the reaction more than others, seen in the graphs below. The 25°C and 45°C reactions had particularly large absorbance spectrum at 420 nm, compared to the rest of the reactions. The 5°C, 15°C, and 35°C reactions did not have smooth curves; instead, had multiple peaks and relatively low absorbances, if any, at 420 nm.