User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/29: Difference between revisions
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== | ==Reaction of OPD and H2O2 in Water with an Mb/Phosphate Catalyst== | ||
* Objectives: | * '''Objectives''': | ||
*1. Continue analysis of reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb catalyst | *1. Continue analysis of reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Phosphate catalyst | ||
*2. Continue synthesis of Na<sub>12</sub>P<sub>2</sub>W<sub>18</sub>O<sub>62</sub> | *2. Continue synthesis of Na<sub>12</sub>P<sub>2</sub>W<sub>18</sub>O<sub>62</sub> | ||
*Procedure for Part 1: | *'''Procedure for Part 1''': | ||
*1. Follow same protocol from [5/20] using Mb in Phosphate buffer with H<sub>2</sub>O as the solvent | *1. Follow same protocol from [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/20#20_May_2013 5/20] using Mb in Phosphate buffer with H<sub>2</sub>O as the solvent | ||
<sub>The Mb did not dissolve in the same fashion as yesterday, when it went into water without vortexing. The solution was centrifuged</sub> | <sub>The Mb did not dissolve in the same fashion as yesterday, when it went into water without vortexing. The solution was centrifuged</sub> | ||
*'''Data''': | |||
[[Image:Mb_Phosphate_H2O_5C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_H2O_5C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br> | |||
[[Image:Mb_Phosphate_H2O_15C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_H2O_15C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br> | |||
[[Image:Mb_Phosphate_H2O_25C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_H2O_25C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br> | |||
[[Image:Mb_Phosphate_H2O_35C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_H2O_35C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br> | |||
[[Image:Mb_Phosphate_H2O_45C_WORKUP_GRAPH.png|375px]] [[Image:Mb_Phosphate_H2O_45C_SEQUENTIAL_WORKUP_GRAPH.png|375px]] <br> | |||
*<b>Observations</b> | |||
*Even though the Mb did not dissolve in water even after centrifugation, there was still a reaction present at higher temperatures. The reaction at 15°C showed a peak between 350-375 nm, which disappeared following the course of the reaction. The reaction at 5°C did not, however, seem to react because of no definite peak at 460 nm; a peak between 350-375 nm was also seen in the final absorbance. | |||
== Synthesis of Na<sub>12</sub>P<sub>2</sub>W<sub>18</sub>O<sub>62</sub>== | |||
*'''Procedure for Part 2''': | |||
*1. After the solution was left to reflux overnight, it was cooled and 100g of KCl was added | |||
*2. Following the addition of KCl, the solution was filtered and the solid was placed in a massed beaker | |||
*3. 200 mL of water was brought to 80°C, and 2x25 mL increments of water were added to the solid until it dissolved (for a total of 50 mL of hot water added) | |||
*4. The mixture was placed in the fridge at 5°C to recrystallize overnight | |||
The protocol for this reaction can be found [http://openwetware.org/wiki/Hartings_AU_Photosynthesis_Lab:Protocols/Na12P2W18O62 here]. | |||
*References: | *References: |
Revision as of 17:34, 8 July 2013
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Reaction of OPD and H2O2 in Water with an Mb/Phosphate Catalyst
The Mb did not dissolve in the same fashion as yesterday, when it went into water without vortexing. The solution was centrifuged
Synthesis of Na12P2W18O62
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