User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/24

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

24 May 2013

  • Objectives:
  • 1. Continue analysis of OPD+H2O2 reaction with Mb in Tris catalyst catalyst at the same temperatures
  • 2. Re-run the reaction with Mb in Citrate to determine if the anomaly peaks were error or if different products were being synthesized


  • Procedure:
  • 1. Followed same procedure as 5/20-5/22 with Mb lyopholized in Tris/Citrate and KCl from 5/22


  • Observations:
  • 1. The Mb in Tris had no anomaly peaks at any temperature
  • 2. The Mb in Tris had the highest absorbances at 5C and 45C
  • 3. The Mb in Citrate did not provide results similar to the previous trials. There was no peak at either 420 nm or 460 nm, and the 45C trial had completely skewed data from the previous temperatures.


Mb in Tris Buffer Wavelength vs. Corrected Absorbance


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/24&oldid=699528"