User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/24: Difference between revisions

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*1. Followed same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/20#20_May_2013 5/20] with Mb lyopholized in Tris/Citrate and KCl from 5/22
*1. Followed same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/20#20_May_2013 5/20] with Mb lyopholized in Tris/Citrate and KCl from 5/22




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The Mb in Tris had no anomaly peaks at any temperature, he Mb in Tris had the highest absorbances at 5C and 45C, and the Mb in Citrate did not produce results similar to the previous trials. There was no peak at either 420 nm or 460 nm, and the 45C trial had completely skewed data from the previous temperatures.
The Mb in Tris had no anomaly peaks at any temperature, he Mb in Tris had the highest absorbances at 5C and 45C, and the Mb in Citrate did not produce results similar to the previous trials. There was no peak at either 420 nm or 460 nm, and the 45C trial had completely skewed data from the previous temperatures.


== Reanalysis of Reaction of Mb Citrate in MeOH ==


[[Image:Mb_Citrate_5C_SEQUENTIAL_May_24_WORKUP.png]]
[[Image:Mb_Citrate_5C_SEQUENTIAL_May_24_WORKUP.png]]

Revision as of 08:24, 30 May 2013

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24 May 2013

  • Objectives:
  • 1. Continue analysis of OPD+H2O2 reaction with Mb in Tris catalyst catalyst at the same temperatures
  • 2. Re-run the reaction with Mb in Citrate to determine if the anomaly peaks were error or if different products were being synthesized


  • Procedure:
  • 1. Followed same procedure as 5/20 with Mb lyopholized in Tris/Citrate and KCl from 5/22


  • Observations:

The Mb in Tris had no anomaly peaks at any temperature, he Mb in Tris had the highest absorbances at 5C and 45C, and the Mb in Citrate did not produce results similar to the previous trials. There was no peak at either 420 nm or 460 nm, and the 45C trial had completely skewed data from the previous temperatures.



Reanalysis of Reaction of Mb Citrate in MeOH



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