User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/24
m (→Reanalysis of Reaction of OPD and H2O2 with Mb/Citrate Catalyst in MeOH)
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Revision as of 19:19, 8 July 2013
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Reaction of OPD and H2O2 n MeOH with Mb/Tris Catalyst
The Mb in Tris had no anomaly peaks at any temperature, he Mb in Tris had the highest absorbances at 5C and 45C, and the Mb in Citrate did not produce results similar to the previous trials. There was no peak at either 420 nm or 460 nm, and the 45C trial had completely skewed data from the previous temperatures.
Reanalysis of Reaction of OPD and H2O2 with Mb/Citrate Catalyst in MeOH
Just found another article about metastable intermediates in myoglobin at low pH. Link is here. The authors discuss that at a low pH, the proximal histidine is protonated, allowing for the uptake of, as they mention, CO, or any other compound. CO may not necessarily be what's binding in this case. If we can determine what products are formed in the reaction, we can infer more about the reactivity of the overall protein and the iron center.
(added on 5/30).
1. Lin et al, Myoglobin content and citrate synthase activity in different parts of the normal human heart, Journal of Applied Physiology, 1990, 69(3), 899-901. Found here.