User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/22: Difference between revisions
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== | ==Reaction of OPD and H2O2 in MeOH with Mb/Phosphate Catalyst== | ||
* Objectives: | * <b>Objectives</b>: | ||
*1. Continue analysis of reaction between OPD and Hydrogen Peroxide with Mb catalyst in Phosphate buffer | *1. Continue analysis of reaction between OPD and Hydrogen Peroxide with Mb catalyst in Phosphate buffer | ||
*2. Lyopholize more solutions of Mb in buffer for reaction | *2. Lyopholize more solutions of Mb in buffer for reaction | ||
*Procedure: | *<b>Procedure</b>: | ||
*1. Same as [5/20/ | *1. Same as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/20#20_May_2013 5/20], except with Phosphate buffer | ||
*<b>Lyopholization of Mb in Buffers</b>: | |||
*1. 5 milligrams of Myoglobin and 490 milligrams of KCl were added to four tubes | |||
*2. 20 mL of each buffer was added (pH 9 Tris; pH 7 Phosphate; pH 5 Acetate; pH 3 Citrate) | |||
*3. The solutions were frozen in liquid nitrogen and lyopholized | |||
*Graphs: | |||
[[Image:5C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:5C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:15C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:15C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:25C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:25C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:35C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:35C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:45C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:45C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
*<b>Observations</b>: | |||
The data was not as consistent as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/21 5/21/2013], especially in the 25°C and 35°C trials. The curves were also wider over the absorbance spectrum, more so than the previous reactions. | |||
Revision as of 16:15, 8 July 2013
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Reaction of OPD and H2O2 in MeOH with Mb/Phosphate Catalyst
The data was not as consistent as 5/21/2013, especially in the 25°C and 35°C trials. The curves were also wider over the absorbance spectrum, more so than the previous reactions.
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