User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/22: Difference between revisions

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*Procedure:
*Procedure:


*1. Same as [5/20/13 and 5/21/13], except with Phosphate buffer
*1. Same as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/20#20_May_2013 5/20], except with Phosphate buffer




Revision as of 06:41, 30 May 2013

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22 May 2013

  • Objectives:
  • 1. Continue analysis of reaction between OPD and Hydrogen Peroxide with Mb catalyst in Phosphate buffer
  • 2. Lyopholize more solutions of Mb in buffer for reaction
  • Procedure:
  • 1. Same as 5/20, except with Phosphate buffer


  • Lyopholization of Mb in Buffers:
  • 1. 5 milligrams of Myoglobin and 490 milligrams of KCl were added to four tubes
  • 2. 20 mL of each buffer was added (pH 9 Tris; pH 7 Phosphate; pH 5 Acetate; pH 3 Citrate)
  • 3. The solutions were frozen in liquid nitrogen and lyopholized
  • Graphs:



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