User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/22

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(22 May 2013)
Current revision (19:15, 8 July 2013) (view source)
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*Graphs:
*Graphs:
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[[Image:5C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
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[[Image:5C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:5C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
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[[Image:15C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
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[[Image:15C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:15C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
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[[Image:25C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
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[[Image:25C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:25C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
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[[Image:35C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
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[[Image:35C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:35C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
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[[Image:45C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
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[[Image:45C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png|375px]] [[Image:45C_Mb_Phosphate_OPD_H2O2_MeOH_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>

Current revision

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Reaction of OPD and H2O2 in MeOH with Mb/Phosphate Catalyst

  • Objectives:
  • 1. Continue analysis of reaction between OPD and Hydrogen Peroxide with Mb catalyst in Phosphate buffer
  • 2. Lyopholize more solutions of Mb in buffer for reaction
  • Procedure:
  • 1. Same as 5/20, except with Phosphate buffer


  • Lyopholization of Mb in Buffers:
  • 1. 5 milligrams of Myoglobin and 490 milligrams of KCl were added to four tubes
  • 2. 20 mL of each buffer was added (pH 9 Tris; pH 7 Phosphate; pH 5 Acetate; pH 3 Citrate)
  • 3. The solutions were frozen in liquid nitrogen and lyopholized
  • Graphs:







  • Observations:

The data was not as consistent as 5/21/2013, especially in the 25°C and 35°C trials. The curves were also wider over the absorbance spectrum, more so than the previous reactions.



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