User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/20: Difference between revisions

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==20 May 2013==
==Reaction of OPD and H2O2 in MeOH with Mb/Citrate Catalyst==


*Objectives:
*Objectives:
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*Procedure [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/Photosynthesis/2013/04/02 Taken from Dr. Hartings' notebook]:


*Procedure:


*1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed
*1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed
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*6. Spectra were then taken at 15C, 25C, 35C, and 45C
*6. Spectra were then taken at 15C, 25C, 35C, and 45C
*7. The data was then analyzed and the spectra were converted to absorbance and corrected
*7. The data was then analyzed and the spectra were converted to absorbance and corrected
*Graphs:
[[Image:Mb_Citrate_5C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_5C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
[[Image:Mb_Citrate_15C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_15C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
[[Image:Mb_Citrate_25C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_25C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
[[Image:Mb_Citrate_35C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_35C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>
[[Image:Mb_Citrate_45C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_45C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br>




Revision as of 13:39, 8 July 2013

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Reaction of OPD and H2O2 in MeOH with Mb/Citrate Catalyst

  • Objectives:
  • 1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate



  • 1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed
  • 2. Added 15 mg of Mb lyopholized in KCl/Citrate to 1.5 mL MeOH and vortexed
  • 3. Added 1.134 mL of 30% hydrogen peroxide to 8.866 mL MeOH
  • 4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes
  • 5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion
  • 6. Spectra were then taken at 15C, 25C, 35C, and 45C
  • 7. The data was then analyzed and the spectra were converted to absorbance and corrected
  • Graphs:








  • Observations:
  • 1. Condensation during the 5 Centigrade spectrum - non-useful data
  • 2. The baseline was much higher during the 15C spectrum - will revisit during data analysis
  • 3. Condensation on the 25C spectrum - take note
  • 4. The spectra had different behaviors and peaks between 300 and 800 nm - need to determine what is being synthesized at these peaks



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