User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/20: Difference between revisions
From OpenWetWare
No edit summary |
Dhea Patel (talk | contribs) |
||
(16 intermediate revisions by 2 users not shown) | |||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Reaction of OPD and H2O2 in MeOH with Mb/Citrate Catalyst== | ||
*Objectives: | *Objectives: | ||
Line 12: | Line 12: | ||
*1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate | *1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate | ||
*Procedure: | |||
*Procedure [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/Photosynthesis/2013/04/02 Taken from Dr. Hartings' notebook]: | |||
*1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed | *1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed | ||
Line 19: | Line 22: | ||
*4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes | *4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes | ||
*5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion | *5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion | ||
*6. Spectra were then taken at 15C, 25C, 35C, and 45C | |||
*7. The data was then analyzed and the spectra were converted to absorbance and corrected | |||
*Graphs: | |||
[[Image:Mb_Citrate_5C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_5C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:Mb_Citrate_15C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_15C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:Mb_Citrate_25C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_25C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:Mb_Citrate_35C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_35C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
[[Image:Mb_Citrate_45C_WORKUP_GRAPH_CORRECTED.png|375px]] [[Image:Mb_Citrate_45C_SEQUENTIAL_WORKUP_GRAPH_CORRECTED.png|375px]] <br> | |||
Line 24: | Line 43: | ||
*Observations: | *Observations: | ||
*1. Condensation during the 5 Centigrade spectrum - non-useful data | *1. Condensation during the 5 Centigrade spectrum - non-useful data | ||
*2. The baseline was much higher during the 15C spectrum - will revisit during data analysis | |||
*3. Condensation on the 25C spectrum - take note | |||
*4. The spectra had different behaviors and peaks between 300 and 800 nm - need to determine what is being synthesized at these peaks | |||
Revision as of 13:39, 8 July 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Reaction of OPD and H2O2 in MeOH with Mb/Citrate Catalyst
|