User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/20: Difference between revisions
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*1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate | *1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate | ||
*Procedure: | *Procedure: | ||
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*4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes | *4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes | ||
*5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion | *5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion | ||
*6. Spectra were then taken at 15C, 25C, 35C, and 45C | *6. Spectra were then taken at 15C, 25C, 35C, and 45C | ||
*7. The data was then analyzed and the spectra were converted to absorbance and corrected | |||
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*2. The baseline was much higher during the 15C spectrum - will revisit during data analysis | *2. The baseline was much higher during the 15C spectrum - will revisit during data analysis | ||
*3. Condensation on the 25C spectrum - take note | *3. Condensation on the 25C spectrum - take note | ||
*4. The spectra had different behaviors and peaks between 300 and 800 nm - need to determine what is being synthesized at these peaks | |||
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