User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/20: Difference between revisions

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*4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes
*4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes
*5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion
*5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion
*6. Spectra were then taken at 15C, 25C, 35C, and 45C




Revision as of 08:08, 20 May 2013

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20 May 2013

  • Objectives:
  • 1. Analyze the reaction of O-phenylenediamine and hydrogen peroxide catalyzed by Mb in KCl/Citrate
  • Procedure:
  • 1. Added 18 mg of O-phenylenediamine to 18 mL of MeOH and vortexed
  • 2. Added 15 mg of Mb lyopholized in KCl/Citrate to 1.5 mL MeOH and vortexed
  • 3. Added 1.134 mL of 30% hydrogen peroxide to 8.866 mL MeOH
  • 4. Centrifuged the Mb solution at 13,200 rpm for 10 minutes
  • 5. The spectrum was taken at 5C, and the H2O2 solution was added at 1% spectrum completion
  • 6. Spectra were then taken at 15C, 25C, 35C, and 45C


  • Observations:
  • 1. Condensation during the 5 Centigrade spectrum - non-useful data



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