User:Paul Rothenberg/Notebook/Pauls Notebook/2013/03/18: Difference between revisions
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= | =18 March 2013== | ||
* | * Objectives: | ||
*1. Run UV-VIS and Fluorescence spectroscopy on prepared solutions of proteins in DMSO | |||
*Procedure: | |||
*1. A control sample of 200 µL DMSO was run in UV-VIS and Fluorescence spectroscopes | |||
*2. 200 µL of Mb, DMSO, Crown, and Phosphate solution was run | |||
*3. 190 µL of DMSO and 10 µL of Mb, DMSO, Crown, Phosphate was run | |||
*4. 199 µL of DMSO and 1 µL of Mb, DMSO, Crown, Phosphate was run | |||
*5. 25 µL of [4] was diluted with 175 µL of DMSO
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*6. 200 µL of Mb, Acetate, DMSO, Crown was run | |||
*7. 20 µL of [6] was diluted with 180 µL DMSO (10x factor) | |||
*8. 20 µL of [7] was diluted with 180 µL DMSO (10x factor) | |||
*Data: | |||
*1. The control spectra showed signs of contamination in the cuvettes; aquaregia was used to clean the cuvettes | |||
*2. DMSO showed a peak between 200-300 nm in UV-VIS spectrum and a large peak in Fluorescence spectroscopy | |||
*3. The 200 µL of DMSO, Crown, Phosphate was too concentrated − diluted | |||
*4. [3] was still too concentrated under fluorescence | |||
*5. [4] was too concentrated − removed and saved; 25 µL of [4] was added along with 175 µL DMSO | |||
*6. Concentration of [5] did not max out the fluorimeter
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*7. [6] was highly concentrated under UV-VIS, and not run under Fluorescence | |||
*8. [7] was too concentrated under fluorescence, and diluted | |||
*9. [8] did not max out the fluorimeter or the UV-VIS | |||
Revision as of 12:11, 18 March 2013
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