User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/04/15: Difference between revisions
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=='''SDS Page Gel Electrophoresis'''== | {|{{table}} width="800" | ||
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->=='''SDS Page Gel Electrophoresis'''== | |||
I. 4 - 12 well pre-cast Mini Protean TGX gel was obtained and prepared. | I. 4 - 12 well pre-cast Mini Protean TGX gel was obtained and prepared. | ||
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Latest revision as of 00:55, 27 September 2017
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SDS Page Gel ElectrophoresisI. 4 - 12 well pre-cast Mini Protean TGX gel was obtained and prepared.
II. The Bio-Rad Mini Protean system electrophoresis cell was assembled.
III. Each of the 4 gels were loaded with each of the 20 μL reaction samples prepared yesterday in the following manner:
IV. The gel was run for approximately 10 min at 200 V, 0.05 A, and 10 W. NOTE: The top of the chamber was not functioning properly in that a closed circuit was not established and the top had to be manually held/pushed down for the entirety of the run. V. After 10 min, the gel was placed in a Fixative Solution (40% methanol, 10% acetic acid, 50% water) for 30 minutes VI. The gel was then placed in a Stain Solution (0.025% (w/v) Coomassie Blue, 10% acetic acid, 90% water) for approximately 24 hours VII.Finally, the gel was placed in Destain Solution (10% acetic acid, 90% water) three times for 15 minutes each and the following results were obtained: |