User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Notes from Protocol Prep== | ==Notes from Protocol Prep== | ||
When trying to resuspend the fibers in solution, they were vortexed gently. The solution became colloidal in color with fibers suspended. When the supernatant was extracted out and replaced with buffer, the fibers did not reconstitute into solution as expected. The solution was sonicated; it appeared that the sonication possibly created a colloidal solution from the fibers. | When trying to resuspend the fibers in solution, they were vortexed gently. The solution became colloidal (purple) in color with fibers suspended. When the supernatant was extracted out and replaced with buffer, the fibers did not reconstitute into solution as expected. The solution was sonicated; it appeared that the sonication possibly created a colloidal solution from the fibers. | ||
Instead, the fibers will be dispersed in the individual test tubes, and concentrated buffer added. This will prevent excess transferring of fibers and dispersion of fibers into colloid. | Instead, the fibers will be dispersed in the individual test tubes, and concentrated buffer added. This will prevent excess transferring of fibers and dispersion of fibers into colloid. | ||
'''The new protocol should be | '''The new protocol should be something similar to this:''' | ||
#Suspend fibers in individual glass tubes | #Suspend fibers in individual glass tubes | ||
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#Add protease solution to the mixture and begin shaking | #Add protease solution to the mixture and begin shaking | ||
#When the reaction is finished, spin the individual tube for 30 seconds and extract the supernatant for analysis | #When the reaction is finished, spin the individual tube for 30 seconds and extract the supernatant for analysis | ||
This should minimize the errors from the protocol we ran today. | |||
Latest revision as of 00:52, 27 September 2017
Project name | Main project page Previous entry Next entry |
New Reaction/Sampling ProtocolHere's the new protocol we developed for analysis:
Notes from Protocol PrepWhen trying to resuspend the fibers in solution, they were vortexed gently. The solution became colloidal (purple) in color with fibers suspended. When the supernatant was extracted out and replaced with buffer, the fibers did not reconstitute into solution as expected. The solution was sonicated; it appeared that the sonication possibly created a colloidal solution from the fibers. Instead, the fibers will be dispersed in the individual test tubes, and concentrated buffer added. This will prevent excess transferring of fibers and dispersion of fibers into colloid. The new protocol should be something similar to this:
This should minimize the errors from the protocol we ran today.
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