User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/17: Difference between revisions
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==100 nM Chymotrypsin In Situ Kinetics== | ==100 nM Chymotrypsin In Situ Kinetics== | ||
10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl<sub>2</sub> buffer. | |||
[[Image:100nM_Chymotrypsin_AbsvsTime_Mar17_Chart.png|500px]] | |||
[[Image:100nM_Chymotrypsin_Kinetics_Mar17_Chart.png|500px]] | |||
=='''Bradford Analysis of Thermolysin Reaction Samples'''== | |||
250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl<sub>2</sub>), and 550 mL of Tris/CaCl<sub>2</sub> buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm. | |||
[[Image:Bradford_kinetics_1uM_thermolysin.png|500px]] | |||
[[Image:Bradford_kinetics_100nM_thermolysin.png|500px]] | |||
[[Image:Bradford_kinetics_10nM_thermolysin.png|500px]] | |||
[[Image:Bradford_kinetics_1nM_thermolysin.png|500px]] | |||
Revision as of 14:47, 31 March 2015
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100 nM Chymotrypsin In Situ Kinetics10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl2 buffer.
Bradford Analysis of Thermolysin Reaction Samples250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl2), and 550 mL of Tris/CaCl2 buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm.
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