User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/04: Difference between revisions
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Switching to Chymotrypsin kinetics. Same protocol for OceanOptics, scan every 30 seconds for 2 hours at 37°C, spinning at 1000 RPM. | Switching to Chymotrypsin kinetics. Same protocol for OceanOptics, scan every 30 seconds for 2 hours at 37°C, spinning at 1000 RPM. | ||
For the solution prep, | For the solution prep, 104 μL of concentrated Chymotrypsin stock (24.00 μM) was diluted by 2.896 mL of Tris-10mM CaCl<sub>2</sub> buffer. | ||
'''Results''' | '''Results''' | ||
Revision as of 08:58, 4 March 2015
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1 μM Chymotrypsin KineticsProtocol Switching to Chymotrypsin kinetics. Same protocol for OceanOptics, scan every 30 seconds for 2 hours at 37°C, spinning at 1000 RPM. For the solution prep, 104 μL of concentrated Chymotrypsin stock (24.00 μM) was diluted by 2.896 mL of Tris-10mM CaCl2 buffer. Results
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