User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/04: Difference between revisions
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== | ==1 μM Chymotrypsin Kinetics== | ||
'''Protocol''' | |||
Switching to Chymotrypsin kinetics. Same protocol for OceanOptics, scan every 30 seconds for 2 hours at 37°C, spinning at 1000 RPM. | |||
For the solution prep, 52.1 μL of concentrated Chymotrypsin stock (47.98 μM) was diluted by 2.9479 mL of Tris-10mM CaCl<sub>2</sub> buffer. | |||
'''Results''' | |||
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1 μM Chymotrypsin KineticsProtocol Switching to Chymotrypsin kinetics. Same protocol for OceanOptics, scan every 30 seconds for 2 hours at 37°C, spinning at 1000 RPM. For the solution prep, 52.1 μL of concentrated Chymotrypsin stock (47.98 μM) was diluted by 2.9479 mL of Tris-10mM CaCl2 buffer. Results
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