User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/25: Difference between revisions

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'''Results'''
'''Results'''
Image:100nM_Tryspin_AbsvsTime_Feb25_Chart.png|500px]]
[[Image:100nM_Tryspin_Kinetics_Feb25_Chart.png|500px]]





Revision as of 17:06, 25 March 2015

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1 µM Trypsin In Situ Kinetics

Protocol

90.7 µL of the stock Trypsin was diluted with 2.909 mL of Tris/NaCl for a final concentration of 1 µM. Fibers were spun yesterday and the liquid was extracted off the top. OceanOptics was run at 2 hours, scanning every 30 seconds.

Results

100 nM Trypsin In Situ Kinetics

Protocol

9.07 µL of the stock was diluted with 2.991 mL Tris/NaCl. Fibers were spun at 1500 RPM inadvertently. Same reaction conditions as above.

Results

Image:100nM_Tryspin_AbsvsTime_Feb25_Chart.png|500px]]