User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/25

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1 µM Trypsin In Situ Kinetics

Protocol

90.7 µL of the stock Trypsin was diluted with 2.909 mL of Tris/NaCl for a final concentration of 1 µM. Fibers were spun yesterday and the liquid was extracted off the top. OceanOptics was run at 2 hours, scanning every 30 seconds.

Results

100 nM Trypsin In Situ Kinetics

Protocol

9.07 µL of the stock was diluted with 2.991 mL Tris/NaCl. Fibers were spun at 1500 RPM inadvertently. Same reaction conditions as above.

Results