User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/12: Difference between revisions
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== | ==Kinetics of 1 nM Proteinase K Degradation of AuNP Fibers== | ||
'''Objectives''' | |||
To continue gathering kinetics data for the protease reaction. | |||
'''Protocol''' | |||
1 nM Proteinase K was made by taking 10 µL of the stock, and diluting up to 1 mL with buffer. 6.9 µL of that was diluted up to 3 mL with buffer for a final reactant volume concentration of 1 nM protease. The fibers were spun at 300 RPM for 10 minutes, and the liquid extracted. The solid fibers were added to the cuvette with minimal liquid. The protease was added on top. | |||
The OceanOptics was set at 37°C, scan every 5 minutes for 6 hours. | |||
'''Results''' | |||
Revision as of 09:24, 12 February 2015
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Kinetics of 1 nM Proteinase K Degradation of AuNP FibersObjectives To continue gathering kinetics data for the protease reaction. Protocol 1 nM Proteinase K was made by taking 10 µL of the stock, and diluting up to 1 mL with buffer. 6.9 µL of that was diluted up to 3 mL with buffer for a final reactant volume concentration of 1 nM protease. The fibers were spun at 300 RPM for 10 minutes, and the liquid extracted. The solid fibers were added to the cuvette with minimal liquid. The protease was added on top. The OceanOptics was set at 37°C, scan every 5 minutes for 6 hours. Results
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