User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/11: Difference between revisions
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The incorrect stock solution was used to make the protease solution. The concentration being tested is not 1 nM. This reaction will be rerun tomorrow with the proper enzyme concentration. | The incorrect stock solution was used to make the protease solution. The concentration being tested is not 1 nM. This reaction will be rerun tomorrow with the proper enzyme concentration. | ||
==Bradford Analysis of Samples from Yesterday== | |||
'''Objectives''' | |||
Bradford analysis of the samples extracted yesterday will be run. | |||
'''Protocol''' | |||
A 1 in 4 Bradford assay was made (1 mL Bradford in 3 mL of Tris/NaCl buffer). | |||
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Revision as of 10:27, 11 February 2015
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In situ 1 nM Proteinase K Kinetics RunObjectives To run the 1 nM concentration of Proteinase K and track over time for kinetics workup. Protocol Take 0.046 mL of dilution from step 4 above in 1.954 mL of Tris/NaCl buffer for a final [proteinase K]final = 10 nM. From there, dilute by a factor of 10 to decrease the final concentration to 1 nM. Ocean Optics will be run at 37°C, scanning every 5 minutes for 6 hours. Results The incorrect stock solution was used to make the protease solution. The concentration being tested is not 1 nM. This reaction will be rerun tomorrow with the proper enzyme concentration.
Bradford Analysis of Samples from YesterdayObjectives Bradford analysis of the samples extracted yesterday will be run. Protocol A 1 in 4 Bradford assay was made (1 mL Bradford in 3 mL of Tris/NaCl buffer). | |
