User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/03: Difference between revisions
From OpenWetWare
Line 52: | Line 52: | ||
==Results== | ==Results== | ||
Proteinase K, Trypsin, and Chymotrypsin all had visible color changes. Pepsin did not have a visible color change. UV-VIS analysis can be found on tomorrow's page. | Proteinase K, Trypsin, and Chymotrypsin all had visible color changes. Pepsin did not have a visible color change. UV-VIS analysis can be found on [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/CHEM_571_Fall_2014/2015/02/04#Objective tomorrow's page]. | ||
Revision as of 17:44, 4 February 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Qualitative Analysis of Protease ActivityObjectiveContinue the qualitative analysis of protease activity on AuNP fibers. Pepsin, Trypsin, Chymotrypsin, and Proteinase K will be tested. ProcedureStock Solutions
Solutions Tested
NB - Pepsin has an optimal pH of 1.5-2, so a pH 2.14, 5 mM Citrate, 0.2 M NaCl buffer was made for Pepsin use. That may affect the results. Reaction Conditions The AuNP fibers were spun down at 300 RPM for 4 minutes. The supernatant was removed, and the prepared solutions were added on top of the solid fibers. The tubes were placed in a shaker at 37°C. They will be analyzed tomorrow. ResultsProteinase K, Trypsin, and Chymotrypsin all had visible color changes. Pepsin did not have a visible color change. UV-VIS analysis can be found on tomorrow's page.
|