User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==SDS== | ||
SDS-PAGE was run on the samples prepared yesterday. The wells were loaded as follows: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''8''' | |||
| align="center" style="background:#f0f0f0;"|'''Colloid''' | |||
|- | |||
| 9||0.6 g/L Lysozyme | |||
|- | |||
| 10||0.12 g/L Lysozyme | |||
|- | |||
| 11||Unknown Sample B | |||
|} | |||
Dr. Fox will finish the workup and present the results, which will be posted upon completion. | |||
==Colloid CaCl<sub>2</sub> Dialysis Analysis== | |||
Analyses to be conducted: | |||
#Bradford | |||
#Fluorescence | |||
#UV-VIS | |||
#Ca ISE | |||
Only Bradford analysis was completed. There was an issue with the blank, but it was resolved. The results are below: | |||
[[Image:Bradford_Colloid_CaCl2_Chart_10_15.png|500px]] | |||
==Dialysis Preparation== | |||
Monika and I prepared the next dialysis for over the weekend. She has the preparation [http://openwetware.org/wiki/User:Monika_Gasiorek/Notebook/CHEM-571_2014F/2014/10/15 here]. It was 3500 MWCO with 0.6 g/L Lysozyme against CaCl<sub>2</sub>. | |||
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Latest revision as of 00:26, 27 September 2017
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SDSSDS-PAGE was run on the samples prepared yesterday. The wells were loaded as follows:
Dr. Fox will finish the workup and present the results, which will be posted upon completion. Colloid CaCl2 Dialysis AnalysisAnalyses to be conducted:
Only Bradford analysis was completed. There was an issue with the blank, but it was resolved. The results are below:
Dialysis PreparationMonika and I prepared the next dialysis for over the weekend. She has the preparation here. It was 3500 MWCO with 0.6 g/L Lysozyme against CaCl2. | |||||||||
