User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/01: Difference between revisions
From OpenWetWare
| Line 12: | Line 12: | ||
[[Image:Bradford_Analysis_Dialysis_10_1.png|500px]] | [[Image:Bradford_Analysis_Dialysis_10_1.png|500px]] | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wavelength''' | |||
| align="center" style="background:#f0f0f0;"|'''575''' | |||
| align="center" style="background:#f0f0f0;"|'''Calculated Concentrations (g/L)''' | |||
|- | |||
| Stock||0.105||0.608040201 | |||
|- | |||
| 50 mM CaCl2||0.108||0.75879397 | |||
|- | |||
| 50 mM CaCl2 Soak||0.012||-4.065326633 | |||
|- | |||
| 500 uM CaCl2||0.103||0.507537688 | |||
|- | |||
| 500 uM CaCl2 Soak||-0.009||-5.120603015 | |||
|- | |||
| HCl||0.092||-0.045226131 | |||
|- | |||
| HCl Soak||-0.005||-4.91959799 | |||
|- | |||
| Water||0.112||0.959798995 | |||
|- | |||
| Water Soak||-0.005||-4.91959799 | |||
|} | |||
A second dialysis was prepared using 2 wells of 0.12 g/L lysozyme and the 30:1 Au:Lysozyme colloid against 20k MW paper. The dialysis will run for a week. | |||
Revision as of 13:49, 1 October 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||
Bradford Analysis of Lysozyme DialysisBradford analysis of the dialysis was run for the soak solution and the protein solution. The results are below, along with calculated concentrations:
| |||||||||||||||||||||||||||||||
