User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/08/27: Difference between revisions

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*Final [Lysozyme] wanted: 250 μM
*Final [Lysozyme] wanted: 250 μM
*Actual amount of Lysozyme:  
*Actual amount of Lysozyme:  
Next, solutions were made with gold:protein ratios of 10:1 to 60:1.
==<font color=#0f1028></font>==
{|style="width:700px"
|<u>Au</u>
|<u>Lysozyme</u>
|<u>H<sub>2</sub>O</u>
|-
|2.5 mL
|2.5 mL
|0 mL
|-
|2.5 mL
|1.25 mL
|1.25 mL
|-
|2.5 mL
|0.835 mL
|1.665 mL
|-
|2.5 mL
|0.625 mL
|1.875 mL
|-
|2.5 mL
|0.5 mL
|2 mL
|-
|2.5 mL
|0.415 mL
|2.08 mL
|-




Revision as of 10:16, 27 August 2014

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Making HAuCl4 and Lysozyme Stocks

  • Final [H+] wanted: 0.25 mM
  • Actual amount of HAuCl4: 0.04550 g
    • (slightly more water added to the volumetric flask)
  • Final [Lysozyme] wanted: 250 μM
  • Actual amount of Lysozyme:


Next, solutions were made with gold:protein ratios of 10:1 to 60:1.

Au Lysozyme H2O
2.5 mL 2.5 mL 0 mL
2.5 mL 1.25 mL 1.25 mL
2.5 mL 0.835 mL 1.665 mL
2.5 mL 0.625 mL 1.875 mL
2.5 mL 0.5 mL 2 mL
2.5 mL 0.415 mL 2.08 mL


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