User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/08/27: Difference between revisions
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0.5 mL of HAuCl<sub>4</sub> stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35. | 0.5 mL of HAuCl<sub>4</sub> stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35. | ||
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! H<sub>2</sub>O | ! H<sub>2</sub>O | ||
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| | | 2.5 mL | ||
| | | 2.5 mL | ||
| | | 0 mL | ||
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| 2.5 mL | |||
| 1.25 mL | |||
| 1.25 mL | |||
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| 2.5 mL | |||
| 0.835 mL | |||
| 1.635 mL | |||
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| 2.5 mL | |||
| 0.625 mL | |||
| 1.875 mL | |||
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| 2.5 mL | |||
| 0.5 mL | |||
| 2 mL | |||
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| 2.5 mL | |||
| 0.415 mL | |||
| 2.08 mL | |||
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Revision as of 11:20, 2 September 2014
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Making HAuCl4 and Lysozyme Stocks
Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00. Kinetics0.5 mL of HAuCl4 stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35.
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