User:Patrick Hampson/Notebook/chem471/2016/11/09: Difference between revisions
| Line 19: | Line 19: | ||
[[Image:BSA_Fluor_pH10_emissionsmax.PNG ]] | [[Image:BSA_Fluor_pH10_emissionsmax.PNG ]] | ||
The graph above displays the flourescence emission intensity over time. There is an initial increase, until around 1500seconds, where a plateau is held right around 425 nm for the remainder of the run. | |||
[[Image:BSA_Fluor_pH10_integratedemissions.PNG]] | [[Image:BSA_Fluor_pH10_integratedemissions.PNG]] | ||
The graph above displays integrated intensity over time. There is an increase until about 2000 seconds, where it peaks at approximately 9000. It then decreases to just under 8000 in the time to 6000 seconds, where it remains for the duration of the experiment. | |||
[[Image:BSA_Fluor_pH10_emissions0-900.PNG]] | [[Image:BSA_Fluor_pH10_emissions0-900.PNG]] | ||
The graph above is the emission intensity over time for our 0-900 second scans. As we have seen in previous trials, this is where largest change in emission intensity occurs around our wavelength of interest. The emission intensity jumps from approximately 10 to over 30 in the 900 seconds span. There also appears to be a minor shift to the right as the experiment went on. | |||
[[Image:BSA_Fluor_pH10_emissions1080-4500.PNG]] | [[Image:BSA_Fluor_pH10_emissions1080-4500.PNG]] | ||
[[Image:BSA_Fluor_pH10_emissions4680-10800.PNG]] | [[Image:BSA_Fluor_pH10_emissions4680-10800.PNG]] | ||
Revision as of 12:44, 9 December 2016
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Fluorescence pH 10
Preparation of UV-VIS samples for next classFructose concentrations of: [0.0625 mM] [0.5 mM] [1.25 mM] pHs 4-12 done for samples of all three concentrations.
The graph above is the emission intensity over time for our 0-900 second scans. As we have seen in previous trials, this is where largest change in emission intensity occurs around our wavelength of interest. The emission intensity jumps from approximately 10 to over 30 in the 900 seconds span. There also appears to be a minor shift to the right as the experiment went on.
| |
