User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2009/03/29: Difference between revisions

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**Number
**Number
==Measurement==
==Measurement==
(Currently available in our lab)
*Bulk GFP signal readout
*Bulk b-gal activity readout
*Bulk Restriction Digestion
*Bulk PCR
*Single-cell GFP readout
(Not available in our lab yet)
*IPTG diffusion
*Bulk Flipper transcription
*Single-cell fipper transcription
*Bulk Flipper translation
*Single-cell flipper translation
*Flipper localizes on the flippee
*in vivo single cell DNA inversion
*Bulk Flippee transcription
*Single-cell Flippee translation


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Revision as of 21:54, 28 March 2009

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Modeling and measurements for flipper operation

  • Insert content here...

Detail Mechanism

  • Inducer IPTG diffuse into a cell
    • Mechanism
    • Rate
    • Number
  • lacI bound IPTG and leave the promoter
    • Mechanism
    • Rate
    • Number
  • Transcription of Flipper
    • Mechanism
    • Rate
    • Number
  • Translation of Flipper
    • Mechanism
    • Rate
    • Number
  • Flipper localizes at Flippee
    • Mechanism
    • Rate
    • Number
  • DNA inversion
    • Mechanism
    • Rate
    • Number
  • Transcription of reporter
    • Mechanism
    • Rate
    • Number
  • Translation of reporter
    • Mechanism
    • Rate
    • Number
  • Detection
    • Mechanism
    • Rate
    • Number

Measurement

(Currently available in our lab)

  • Bulk GFP signal readout
  • Bulk b-gal activity readout
  • Bulk Restriction Digestion
  • Bulk PCR
  • Single-cell GFP readout

(Not available in our lab yet)

  • IPTG diffusion
  • Bulk Flipper transcription
  • Single-cell fipper transcription
  • Bulk Flipper translation
  • Single-cell flipper translation
  • Flipper localizes on the flippee
  • in vivo single cell DNA inversion
  • Bulk Flippee transcription
  • Single-cell Flippee translation


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