User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/28: Difference between revisions
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(Autocreate 2008/10/28 Entry for User:Pakpoom_Subsoontorn/Notebook/Genetically_Encoded_Memory) |
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== | ==PhiC31== | ||
* | *Host: Streptomyces lividans | ||
*Action: tyrosine integrase | |||
*Structure: | |||
length:: 613 aa | |||
crystal structure is unknown. | |||
*Target sequence: attB:: has two inverted repeated sequence (core-type) flanking an overlap region (TTG). Minimal attB is 34 bps while minimal attP is 39 bp for e.coli intramolecular integration. | |||
attP:: has core-type sites and overlap region, without an arm-type site | |||
*Change specificity: | |||
Mutation/directed evolution could change specificity to pseudo attP site (HpsA, 44% identity) | |||
*Mechanism: | |||
*Excision: | |||
Excisionase has not yet been found. | |||
*Usage: | |||
Integration works in E.coli, mammalian and in vitro | |||
Revision as of 02:54, 9 November 2008
 Project name
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PhiC31
length:: 613 aa crystal structure is unknown.
attP:: has core-type sites and overlap region, without an arm-type site
Mutation/directed evolution could change specificity to pseudo attP site (HpsA, 44% identity)
Excisionase has not yet been found.
Integration works in E.coli, mammalian and in vitro
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