User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/16: Difference between revisions

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** integrase
** integrase
** excisionase
** excisionase
*
** Tyrosine-recombinase, Serine recombinase (comparison)
* Engineering perspective: what do engineer wanna know
** reversibility/ efficiency
** specificity
** speed
** modularity (for protein engineer)
* The Database project..for site-specific recombinase..and maybe other DNA-writer
* The Database project..for site-specific recombinase..and maybe other DNA-writer


Revision as of 19:20, 29 October 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Writing to DNA

  • Insert content here...

Outline

  • Goal: To change to sequence of DNA at a specific locations
  • Approaches:
    • Site-specific double-stranded break
    • Error-prone DNA polymerase
    • Site-specific recombinases
      • Classification by functions (analogous): transposase, invertase, resolvase, integrase
      • Classification by structures (homologous): tyrosine recombinase, serine recombinase
  • Site-specific double-stranded break
    • DSB activate repairing mechanism, especially homologous recombination
    • custom-made Zn-finger binding protein
  • Error-prone DNA polymerase
    • targeted by ORI on plasmid
    • invivo directed evolution
  • Site-specific recombinase: by function and by structures
    • transposase
    • invertase
    • resolvase
    • integrase
    • excisionase
    • Tyrosine-recombinase, Serine recombinase (comparison)
  • Engineering perspective: what do engineer wanna know
    • reversibility/ efficiency
    • specificity
    • speed
    • modularity (for protein engineer)
  • The Database project..for site-specific recombinase..and maybe other DNA-writer



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