User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/14: Difference between revisions

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==Tools==
==Tools==
* We probably want to have standard cassettes on plasmids so that we can try wide varieties of recombination sites and recombination enzymes
* Standard cassettes on plasmids so that we can try wide varieties of recombination sites and recombination enzymes
* Tunable promoter and some reporter tags that report the levels of recombinase
* Method for timing the recombination process. At what time point the recombination of DNA is complete? This could time scale could be much shorter than the time until the reporter of the new DNA configuration become observable. Can we pause/terminate recombination at different time point?
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Revision as of 19:55, 16 October 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Quantitative functional profiles of site-specific recombinase

  • Insert content here...

Quantitative functional information

Here are the list things we want to measure and compare:

  • Recombination efficiency
  • Recombination speed

Here are some parameter we want to tune,

  • Enzyme concentration
  • Expression time

Tools

  • Standard cassettes on plasmids so that we can try wide varieties of recombination sites and recombination enzymes
  • Tunable promoter and some reporter tags that report the levels of recombinase
  • Method for timing the recombination process. At what time point the recombination of DNA is complete? This could time scale could be much shorter than the time until the reporter of the new DNA configuration become observable. Can we pause/terminate recombination at different time point?


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