User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==miniProject: Expanding recombinase library==
==miniProject: Expanding recombinase library==
* For this project, we will evolve a large library of integrase/excisionase system whose att sequences do not exist in nature. The experiment will involve protein evolution, high-throughput screening assay and bioinformatics. We also want to learn what set of sequence cannot be used as integrase target.
* For this project, we will evolve a large library of recombinase system whose target sequences do not exist in nature. The experiment will involve protein evolution, high-throughput screening assay and bioinformatics. We also want to learn what set of sequence cannot be used as a target.
* Goal: higher efficiency, irreversibility, change of site-specificity
* Goal: higher efficiency, irreversibility, change of site-specificity


Revision as of 20:24, 16 October 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

miniProject: Expanding recombinase library

  • For this project, we will evolve a large library of recombinase system whose target sequences do not exist in nature. The experiment will involve protein evolution, high-throughput screening assay and bioinformatics. We also want to learn what set of sequence cannot be used as a target.
  • Goal: higher efficiency, irreversibility, change of site-specificity

Project outline

Experiment setup

Questions

  • What're the general limitations of protein evolution? What could be the particular limitations in evolving recombinases? Library site? Screening?

TO DO list

Reference


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Pakpoom_Subsoontorn/Notebook/Genetically_Encoded_Memory/2008/10/11&oldid=252879"