User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/10: Difference between revisions
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Revision as of 03:00, 9 November 2008
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Summary Note on site-specific recombinase system
ClassificationKeywords for classification, which can be overlapped
Methods
Site Specific recombinases(see more experiment detail)
attB:: has two inverted repeated sequence flanking an overlap region. inverted repeated sequence (core-type). The overlap region shares sequence with attP. Crossing over occurs at the overlap region. overlap-region::TTCGTAA attP has arm-type sites flanking core-type site.
IHF is required.
Name: HP1, Host: Haemophilus influenzae
length:: 337 aa crystal structure of C-terminal catalytic domain is known
attB:: has two inverted repeated sequence flanking an overlap region. inverted repeated sequence (core-type). The overlap region shares sequence with attP. Crossing over occurs at the overlap region. overlap-region::TTTTAAA attP has arm-type sites flanking core-type site.
Name: L5, Host: Mycobacterium smegmatis
length:: 469 aa crystal structure is unknown.
attP:: has core-type sites and overlap region, without an arm-type site. Minimal attB and attP site is <56 bps.
Excisionase has not yet been found.
Integration works in mammalian cell.
length:: 485 aa. crystal structure is unknown.
attP:: has core-type sites and overlap region, without an arm-type site. Minimal attB and attP site is <56 bps
Integration works in E.coli, mammalian cell and in vitro.
Name: FLP, Host: Saccharomyces cerevisiae
Name: Tn3, Host: Klebsiella pneumoniae Action: resolvase
questions/notes
DatabaseReference
improved genomic integration at a native human sequence. Nucl Acids Res, 29 5044-5051
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