User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/05: Difference between revisions
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==Science Questions/ To-do list== | ==Science Questions/ To-do list== | ||
* | * These are some ideas about reversible genetic knockout | ||
* Can we have a system of two pieces of DNA that can fuse and re-split? The fusion is triggered by signal-1, splitting is triggered by signal-2. Well, this will be similar to the way phages enter and exit their lysogenic cycle. Now, the challenges are the following. First, unlike natural phage, the split of DNA must not lead to the lytic cycle. We want to host to survive. Second, the host has to maintain the two separated pieces of DNA. Third, the fusion and splitting efficiency must be high. | |||
* Can we modify integrase system to function like transposon (but higher specificity/ efficiency)? The piece of jumping DNA of attP for two different integrase systems. Signal-1 makes it insert to attB-1; signal-1' cuts it out; signal-2 makes it insert at attB; signal-2' cut it out again. | |||
==References== | ==References== |
Revision as of 23:59, 5 October 2008
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Thought
Management Questions/requests
audiences? or just leave it as a note among ourselves?
Science Questions/ To-do list
References |