User:Nzimm: Difference between revisions
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| XI || Modify residues to allow proteins other than p8 to embed in the membrane and serve as the phage filament coat | | XI || Modify residues to allow proteins other than p8 to embed in the membrane and serve as the phage filament coat | ||
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Revision as of 00:52, 27 February 2007
Neil Zimmerman
Bio
- MIT Class of 2009
- Major: Biological Engineering
- Minors: Management and Music
- Varsity Soccer
- Wind Ensemble (Clarinet)
20.109
M13K07 Genome Engineering Plans:
| Gene | Plans |
|---|---|
| I | Change # of protein copies expressed to experiment with different sized channels |
| II | Modify residues to allow deactivation of p2 under certain conditions so that replication of + strand can be regulated |
| III | Insert myc to allow detection with an antibody |
| IV | Change # of protein copies expressed to experiment with different sized channels |
| V | Add fluorescent tag to monitor levels of p5-ssDNA complex |
| VI | Modify residues to help p3 bind more effectively to the ToIA protein on the E. coli F pilus |
| VII | Minimize the bulk of the protein to allow more room for modifications on p9 |
| VIII | Insert myc (as in III) or another tag to serve as a “hook” for attaching constructs to M13 |
| IX | Add residues to N-terminus to present on the outside of the phage coat |
| X | Add sensitivity to different stimulus than that of p2 in order to regulate replication of + strand in another fashion |
| XI | Modify residues to allow proteins other than p8 to embed in the membrane and serve as the phage filament coat |
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Email: nzimm (at) mit (dot) edu
