#Establishment and application of new genetic engineering tools and expression/secretion systems in ''Bacillus subtilis'', ''Geobacillus'' and ''Brevibacillus brevis''.
#Biomass saccharification for productions of biofuels and bioproducts.
#Metabolism and metabolic engineering of ''Bacillus subtilis''.
#Tunable physiochemical properties of lignocellulosic biomass via chemical treatments
#Enzyme Engineering by directed evolution: A novel powerful and highly efficient ''Bacillus subtilis'' platform for cellulase engineering and performance improvement towards natural cellulose substrate has been established.
#Acid/base functionalized solid catalysts for hydrolysis and hydrogenation of biomass components to specialty chemicals
#Construction of recombinant consolidated bioprocessing (CBP) microorganisms (e.g., ''Bacillus'', ''Geobacillus'' and yeast) for one-step production of biocommodities from renewable lignocellulosic materials.
#Synthetic biology and metabolic engineering for biofuel production from biomass.
#Microbiological resources and ''in situ'' biodegradation.
Noppadon Sathitsuksanoh (Tik)
Ph.D. candidate
200 Seitz Hall
Biological Systems Engineering Department
Virginia Tech
Blacksburg, VA 24061
sathino@vt.edu
Primary Research
Biomass saccharification for productions of biofuels and bioproducts.
Tunable physiochemical properties of lignocellulosic biomass via chemical treatments
Acid/base functionalized solid catalysts for hydrolysis and hydrogenation of biomass components to specialty chemicals
Selected Publications
Zhang, X.-Z., Sathitsuksanoh, N.,Zhu, Z. and Zhang, Y.-H. P. (2011) One-step production of lactate from cellulose as sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis, under review.
Myung, S., Zhang, X.-Z., and Zhang, Y.-H. P. (2011) Ultra-stable phosphoglucose isomerase through immobilization of cellulosebinding module-tagged thermophilic enzyme on low-cost high-capacity cellulosic adsorbent, Biotechnology Progress In Press
Zhang, X.-Z., and Zhang, Y.-H. P. (2011) Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis, Microbial Biotechnology 4, 98-105.
Zhang, X.-Z., Zhang, Z., Zhu, Z., Sathitsuksanoh, N., Yang, Y., and Zhang, Y.-H. P. (2010) The non-cellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity, Applied Microbiology and Biotechnology 86, 525-533.
Zhang, X.-Z., and Zhang, Y.-H. P. (2010) One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges, Engineering in Life Sciences 10(5):398-406.
Zhang, X.-Z., Sathitsuksanoh, N., and Zhang, Y.-H. P. (2010) Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: heterologous expression, characterization, and synergy with family 48 cellobiohydrolase, Bioresource Technology 101, 5534-5538.
Liu, W., Zhang, X.-Z., Zhang, Z., and Zhang, Y.-H. P. (2010) Engineering of Clostridium phytofermentans endoglucanase Cel5A for improved thermostability, Appl. Environ. Microbiol. 76, 4914-4917.
Zhang, X.-Z., Yan, X., Cui, Z. L., Hong, Q., and Li, S. P. (2006) mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis, Nucleic Acids Res 34, e71.
Zhang, X.-Z., Cui, Z.-L., Hong, Q., and Li, S.-P. (2005) High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800, Appl. Environ. Microbiol. 71, 4101-4103.
Cui, Z.-L., Zhang, X.-Z., Zhang, Z.-H., and Li, S.-P. (2004) Construction and application of a promoter trapping vector with methyl parathion hydrolase gene mpd as the reporter, Biotechnology Letters 26, 1115-1118.
