User:Nikolai Eroshenko

Research

I am building new technologies for basic and applied biology. Long-term research interests include:

• human transgene vectors that integrate genes into precise locations on the genome
• cheaper, faster, and more accurate gene synthesis
• library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions

Publications

Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)

Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy 19:469 (2012)

• Supplementary Data

Gene assembly from chip-synthesized oligonucleotides (PDF)

Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4:1 (2012)

• Gene Assembly by Subpool PCR (GASP) oligo design scripts and server

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF)

Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)

• Supplementary Text and Figures (.pdf)
• Supplementary Sequences (.xls)
• Commentary: Nature Biotechnology; Nature Reviews Genetics]

Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)

Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)

Patents

Orthogonal amplification and assembly of nucleic acid sequences, PCT/US2011/057075, pending

Labs

George Church (2009-now)

Wyss Institute (2009-2011)

Raj Rao (2006-2008)

Curtis Taylor (2006-2007)

Education

Harvard University (2008-now) PhD candidate, Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail

Nikolai Eroshenko
77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115