User:Nikolai Eroshenko: Difference between revisions

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== Research ==
==Education==
[http://www.seas.harvard.edu/academics/graduate Harvard University] (2008-now) PhD candidate, Engineering Sciences: Bioengineering
 
[http://www.egr.vcu.edu/departments-centers/biomedical-engineering/ Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
 
==Contact==
eroshenkona AT gmail


I am interested in developing techniques for massively parallel analyses of DNA-binding proteins and using such methods to study the principles that govern the evolution of transcription factors. I am also interested in designing enzymes capable of efficient and accurate gene delivery.
77 Ave. Louis Pasteur<br>
NRB 238 <br>
Boston, MA 02115


== Publications [http://scholar.google.com/citations?user=FxBA_wQAAAAJ&hl=en google scholar citations]==
== Research Interests ==
6. '''Nikolai Eroshenko''', George Church (2013) [http://www.nature.com/ncomms/2013/130923/ncomms3509/full/ncomms3509.html Mutants of Cre recombinase with improved accuracy.] Nat Commun 4:2509
synthetic evolution, gene therapy, gene synthesis, origins of life, protein design
 
== Publications ==
6. '''Nikolai Eroshenko''', George Church (2013) [http://arep.med.harvard.edu/pdf/Eroshenko_rec_13.pdf Mutants of Cre recombinase with improved accuracy.] Nat Commun 4:2509


5. '''Nikolai Eroshenko'''*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) [http://www.jbioleng.org/content/pdf/1754-1611-7-7.pdf Effect of substrate stiffness on early human embryonic stem cell differentiation.] J Biol Eng 7:7
5. '''Nikolai Eroshenko'''*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) [http://www.jbioleng.org/content/pdf/1754-1611-7-7.pdf Effect of substrate stiffness on early human embryonic stem cell differentiation.] J Biol Eng 7:7
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==Patents==
==Patents==
1. George Church, Sriram Kosuri, '''Nikolai Eroshenko''' (pending) [http://patentscope.wipo.int/search/docservicepdf_pct/id00000019172253.pdf Orthogonal amplification and assembly of nucleic acid sequences] US Patent App 2011057075 (Harvard)
1. George Church, Sriram Kosuri, '''Nikolai Eroshenko''', [http://patentscope.wipo.int/search/docservicepdf_pct/id00000019172253.pdf Orthogonal amplification and assembly of nucleic acid sequences] International Patent Application No. PCT/US2011/05707 (Harvard)


==Labs==
== News ==
[http://arep.med.harvard.edu/ George Church] (2009-now)
[http://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2708.html Highly accurate Cre variants], Nature Methods, Oct. 30 2013


[http://wyss.harvard.edu/ Wyss Institute] (2009-2011)
[http://www.biotechniques.com/news/Refining-a-Recombinase/biotechniques-347492.html#.UmV-fZSifTw Refining a Recombinase], BioTechniques, Oct. 20 2013, Jeffrey M. Perkel


[http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao] (2006-2008)
[http://www.nature.com/nrg/journal/v12/n1/full/nrg2924.html Technology: Making more genes, better and for less], Nature Reviews Genetics, Jan. 2011, Louisa Flintoft


[http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor] (2006-2007)
[http://www.nature.com/scibx/journal/v4/n1/full/scibx.2011.28.html Gene construction using microchip-based DNA synthesis and PCR amplification], SciBX, Jan. 6 2011,


==Education==
[http://www.nature.com/nbt/journal/v28/n12/full/nbt.1723.html Megabases for kilodollars], Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman
[http://seas.harvard.edu/ Harvard University] (2008-now) PhD candidate, Bioengineering
 
[http://www.egr.vcu.edu/Page.aspx?id=93 Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
 
==Contact==
eroshenkona AT gmail
 
 
Nikolai Eroshenko <br>
77 Ave. Louis Pasteur<br>
NRB 238 <br>
Boston, MA 02115

Revision as of 07:44, 23 May 2014

Education

Harvard University (2008-now) PhD candidate, Engineering Sciences: Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail

77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115

Research Interests

synthetic evolution, gene therapy, gene synthesis, origins of life, protein design

Publications

6. Nikolai Eroshenko, George Church (2013) Mutants of Cre recombinase with improved accuracy. Nat Commun 4:2509

5. Nikolai Eroshenko*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) Effect of substrate stiffness on early human embryonic stem cell differentiation. J Biol Eng 7:7

4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors. Gene Ther 19:469

3. Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) Gene assembly from chip-synthesized oligonucleotides. Curr Prot Chem Biol 4:1

2. Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol 28:1295

1. Sheena Abraham, Nikolai Eroshenko & Raj R. Rao (2009) Role of bioinspired polymers in determination of pluripotent stem cell fate. Regen Med 4:561

Patents

1. George Church, Sriram Kosuri, Nikolai Eroshenko, Orthogonal amplification and assembly of nucleic acid sequences International Patent Application No. PCT/US2011/05707 (Harvard)

News

Highly accurate Cre variants, Nature Methods, Oct. 30 2013

Refining a Recombinase, BioTechniques, Oct. 20 2013, Jeffrey M. Perkel

Technology: Making more genes, better and for less, Nature Reviews Genetics, Jan. 2011, Louisa Flintoft

Gene construction using microchip-based DNA synthesis and PCR amplification, SciBX, Jan. 6 2011,

Megabases for kilodollars, Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman