User:Nikolai Eroshenko

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== Research ==
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==Education==
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[http://www.seas.harvard.edu/academics/graduate Harvard University] (2008-now) PhD candidate, Engineering Sciences: Bioengineering
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[http://www.egr.vcu.edu/departments-centers/biomedical-engineering/ Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
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==Contact==
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eroshenkona AT gmail
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77 Ave. Louis Pasteur<br>
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NRB 238 <br>
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Boston, MA 02115
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I am building new technologies for basic and applied biology. Long-term research interests include:
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== Research Interests ==
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* human transgene vectors that integrate genes into precise locations on the genome
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gene therapy; gene synthesis; origins of life; protein selection and design; evolution of complexity
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* cheaper, faster, and more accurate gene synthesis
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* library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
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== Publications ==
== Publications ==
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6. '''Nikolai Eroshenko''', George Church (2013) [http://arep.med.harvard.edu/pdf/Eroshenko_rec_13.pdf Mutants of Cre recombinase with improved accuracy.] Nat Commun 4:2509
5. '''Nikolai Eroshenko'''*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) [http://www.jbioleng.org/content/pdf/1754-1611-7-7.pdf Effect of substrate stiffness on early human embryonic stem cell differentiation.] J Biol Eng 7:7
5. '''Nikolai Eroshenko'''*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) [http://www.jbioleng.org/content/pdf/1754-1611-7-7.pdf Effect of substrate stiffness on early human embryonic stem cell differentiation.] J Biol Eng 7:7
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4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, '''Nikolai Eroshenko''', Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) [http://www.nature.com/gt/journal/v19/n5/full/gt2011134a.html Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors.] Gene Ther 19:469
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4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, '''Nikolai Eroshenko''', Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) [https://dl.dropboxusercontent.com/u/5389110/gt2011134a.pdf Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors.] Gene Ther 19:469
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3. '''Nikolai Eroshenko'''*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) [http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf Gene assembly from chip-synthesized oligonucleotides.] Curr Prot Chem Biol 4:1
3. '''Nikolai Eroshenko'''*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) [http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf Gene assembly from chip-synthesized oligonucleotides.] Curr Prot Chem Biol 4:1
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2. Sriram Kosuri*, '''Nikolai Eroshenko'''*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) [http://dl.dropbox.com/u/5389110/nbt.1716.pdf Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips.] Nat Biotechnol 28:1295
2. Sriram Kosuri*, '''Nikolai Eroshenko'''*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) [http://dl.dropbox.com/u/5389110/nbt.1716.pdf Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips.] Nat Biotechnol 28:1295
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1. Sheena Abraham, '''Nikolai Eroshenko''' & Raj R. Rao (2009) [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf Role of bioinspired polymers in determination of pluripotent stem cell fate.] Regen Med 4:561
1. Sheena Abraham, '''Nikolai Eroshenko''' & Raj R. Rao (2009) [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf Role of bioinspired polymers in determination of pluripotent stem cell fate.] Regen Med 4:561
==Patents==
==Patents==
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1. George Church, Sriram Kosuri, '''Nikolai Eroshenko''' (pending) [http://patentscope.wipo.int/search/docservicepdf_pct/id00000019172253.pdf Orthogonal amplification and assembly of nucleic acid sequences] US Patent App 2011057075 (Harvard)
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1. George Church, Sriram Kosuri, '''Nikolai Eroshenko''', [http://patentscope.wipo.int/search/docservicepdf_pct/id00000019172253.pdf Orthogonal amplification and assembly of nucleic acid sequences] International Patent Application No. PCT/US2011/05707 (Harvard)
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==Labs==
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== News ==
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[http://arep.med.harvard.edu/ George Church] (2009-now)
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[http://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2708.html Highly accurate Cre variants], Nature Methods, Oct. 30 2013
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[http://wyss.harvard.edu/ Wyss Institute] (2009-2011)
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[http://www.biotechniques.com/news/Refining-a-Recombinase/biotechniques-347492.html#.UmV-fZSifTw Refining a Recombinase], BioTechniques, Oct. 20 2013, Jeffrey M. Perkel
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[http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao] (2006-2008)
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[http://www.nature.com/nrg/journal/v12/n1/full/nrg2924.html Technology: Making more genes, better and for less], Nature Reviews Genetics, Jan. 2011, Louisa Flintoft
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[http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor] (2006-2007)
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[http://www.nature.com/scibx/journal/v4/n1/full/scibx.2011.28.html Gene construction using microchip-based DNA synthesis and PCR amplification], SciBX, Jan. 6 2011,
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==Education==
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[http://www.nature.com/nbt/journal/v28/n12/full/nbt.1723.html Megabases for kilodollars], Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman
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[http://seas.harvard.edu/ Harvard University] (2008-now) PhD candidate, Bioengineering
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[http://www.egr.vcu.edu/Page.aspx?id=93 Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
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==Contact==
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eroshenkona AT gmail
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Nikolai Eroshenko <br>
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77 Ave. Louis Pasteur<br>
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NRB 238 <br>
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Boston, MA 02115
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Current revision

Contents

Education

Harvard University (2008-now) PhD candidate, Engineering Sciences: Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail

77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115

Research Interests

gene therapy; gene synthesis; origins of life; protein selection and design; evolution of complexity

Publications

6. Nikolai Eroshenko, George Church (2013) Mutants of Cre recombinase with improved accuracy. Nat Commun 4:2509

5. Nikolai Eroshenko*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) Effect of substrate stiffness on early human embryonic stem cell differentiation. J Biol Eng 7:7

4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors. Gene Ther 19:469

3. Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) Gene assembly from chip-synthesized oligonucleotides. Curr Prot Chem Biol 4:1

2. Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol 28:1295

1. Sheena Abraham, Nikolai Eroshenko & Raj R. Rao (2009) Role of bioinspired polymers in determination of pluripotent stem cell fate. Regen Med 4:561

Patents

1. George Church, Sriram Kosuri, Nikolai Eroshenko, Orthogonal amplification and assembly of nucleic acid sequences International Patent Application No. PCT/US2011/05707 (Harvard)

News

Highly accurate Cre variants, Nature Methods, Oct. 30 2013

Refining a Recombinase, BioTechniques, Oct. 20 2013, Jeffrey M. Perkel

Technology: Making more genes, better and for less, Nature Reviews Genetics, Jan. 2011, Louisa Flintoft

Gene construction using microchip-based DNA synthesis and PCR amplification, SciBX, Jan. 6 2011,

Megabases for kilodollars, Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman

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