User:Nicole Bonan/Notebook/Chem 571 Lab Notebook/2015/09/30
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ObjectiveThe purpose of today's lab work is to:
ProtocolDr. Hartings's protocol for today is here. We are following his protocols for fluorescence and for the Bradford Assay. Fluorescence Analysis of Protease DegradationYesterday, we measured the fluorescence of all of our samples of alpha-chymotrypsin + lysozyme. Today, we finished the fluorescence measurements for the alpha-chymotrypsin blanks as follows:
Brasford Analysis of Protease DegradationData and AnalysisFluorescenceThe above figure shows the fluorescence of lysozyme in samples of varying concentrations of alpha-chymotrypsin as a function of the wavelength of incident light. The fluorescence of each sample was corrected for the blanks by subtracting the fluorescence of the blank from the fluorescence of the sample for every wavelength measured. Each curve on the graph represents a different concentration of alpha-chymotrypsin. The above image shows the fluorescence intensity of lysozyme as a function of the concentration of alpha-chymotrypsin that it is reacted with. Bradford Assay |