User:Nicole Bonan/Notebook/Chem 571 Lab Notebook/2015/09/30

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Objective

The purpose of today's lab work is to:

  1. Finish taking fluorescence data from yesterday's lab
  2. Use a Bradford Assay in order to measure alpha-chymotrypsin degradation as a function of incubation time

Protocol

Dr. Hartings's protocol for today is here. We are following his protocols for fluorescence and for the Bradford Assay.

Fluorescence Analysis of Protease Degradation

Yesterday, we measured the fluorescence of all of our samples of alpha-chymotrypsin + lysozyme. Today, we finished the fluorescence measurements for the alpha-chymotrypsin blanks as follows:



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