User:Nathan H. Kipniss/Notebook/20.109 Final Project: Difference between revisions
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==Elucidating the function of D24H Inverse Pericam mutant== | ==Elucidating the function of D24H Inverse Pericam mutant== | ||
==Background== | |||
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes. | |||
==Ideas== | |||
-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work) | |||
- consider looking at calmodulin only. | |||
Revision as of 18:55, 30 April 2012
Elucidating the function of D24H Inverse Pericam mutant
Background
In module 2 of 20.109, we created the D24H mutant of inverse pericam. The experimental results from the calcium binding assay was surprising as calcium affinity decreased (Kd increase), yet cooperativtiy increased. For a final research idea, I would like to propose the set of experiments that would elucidate how the addition of a histidine into the first binding loop of inverse pericam can make these changes.
Ideas
-working under the assumption that the SDM did indeed work (sequencing with BLAST, discontinuous mega-blast suggests it did indeed work)
- consider looking at calmodulin only.
