User:Nancy T. Miles/Notebook/N.Miles Lab 2013-09-03/2013/09/24
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ObjectiveTo observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. Procedure
The experimental details (pepsin cleavage of hemoglobin) are similar to this reference. We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
DataThe UV-Vis absorbance was measured for each of the aliquoted samples from this experiment. The following spectra are the results: |