Nanofibers with Doubled Concentrations
Objectives
- New nanofibers were made with doubled concentrations with the same 100:1 gold:myoglobin raio.
- Begin retention tests
Methods
- Gather data
- Print 7 more scaffolds and make more nanofibers according to yesterday's procedure
"Brute Force" method
- For each nanofiber solution, there is one third of the tall scaffold
- Place one tube worth of nanofibers on one of the thirds (repeat for other eight scaffold thirds)
- Allow nanofibers to dry on the scaffolds overnight
Retention tests
- Put coated scaffold in PBS buffer
- Put coated scaffold in ethanol
- Put coated scaffold in water at 35C for 30min
- Put coated scaffold in water and sonicate for 30min
- Put coated scaffold in 0.1M HCl solution
- Put coated scaffold in water
- Pictures will be taken of sonicated and 35C after sonication and heating are complete
- Pictures will be taken of all solutions approximately every 2 days (Friday, Monday, and Wednesday)
Results
- 100:1 Au:Myoglobin nanofibers
- 100:1 Au:Myoglobin nanofibers on 4 tall scaffold
- 100:1 Au:Myoglobin nanofibers on 1 extra tall scaffold
- Sonicated samples
|