User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/29: Difference between revisions
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==Procedure 2== | ==Procedure 2== | ||
# 5ml of LB medium was added to two set of sterile tubes by 10 ml pipets | |||
# 5ul of Ampicillin was added to both test tubes | |||
# The frozen cells of PQE-80 bacteria in glycerol were taken out of the -80 degree C fridge | |||
# Some PQE-80 bacteria cells were added to these LB medium under sterile conditions | |||
# PQE-80 bacteria were also added to four erlyn meyer flasks containing LB media and 35ul Ampicillin | |||
# The tubes and flasks were placed in the incubator/shaker at 37 degree C for 24h | |||
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Revision as of 06:55, 5 February 2013
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Entry titlePreparing protein solutions and lyophilization Objective 1Eight different protein solutions containing were prepared for lyophilization Procedure 1
Objective 2Culturing PQE-80 antibiotic resistant bacteria Procedure 2
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