User:Morgan G. I. Langille/Notebook/Halophiles/2010/08/09: Difference between revisions

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==Genome Rearrangements in Hfx. mediterranei==
==Genome Rearrangements in Hfx. mediterranei==
* JE forwarded an email from Adit Naor in reference to PCR product suggesting that inversion of large region in Hfx. mediterranei shown in sequencing is not real. [[Media:Mediterranei.pdf‎|Email from Adit Naor (converted to pdf)]]
* JE forwarded an email from Adit Naor in reference to PCR product suggesting that inversion of large region in Hfx. mediterranei shown in sequencing is not real.
** JE and AD referred me to [[PMID:7868620|Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei]] paper that showed the same inversion that we found with sequencing.  
** JE and AD referred me to [[PMID:7868620|Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei]] paper that showed the same inversion that we found with sequencing.  
** Possible explanations to why they got their PCR result:
** Possible explanations to why they got their PCR result:

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Genome Rearrangements in Hfx. mediterranei

  • JE forwarded an email from Adit Naor in reference to PCR product suggesting that inversion of large region in Hfx. mediterranei shown in sequencing is not real.
    1. They have a different strain and the inversion could have happened recently
    2. The inversion is not stable and both could be present in the population (mate pairs in sequence data actually might support both arrangements of the genome)
    3. PCR experiment might not be valid. Are the primers unique enough? Negative control?



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