User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/28: Difference between revisions
(fix raw html notebook nav) |
|||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 62: | Line 62: | ||
Reaction time: 6 days | Reaction time: 6 days | ||
All 8 reactions were put on . | |||
All 8 reactions were put on the incubator shaker set to 37°C. | |||
Approximately 2 hours into the reaction, no visible color change was observed. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 00:41, 27 September 2017
Project name | Main project page Previous entry Next entry |
January 28th, 2015Pepsin Reaction PreparationThe 50 - 5 mL samples of AuNPs were observed for the formation of fiber aggregates. The presence of these fibers was confirmed. 8 of these 5 mL samples were taken and the supernatant solution was drained using a disposable pipet. Not all 5 mL of solution was removed from each test tube --> While majority of the fibers stuck to the walls of the glass tubes, some were free in solution and were susceptible to being sucked up with the supernatant. NOTE: Concentrations of pepsin were thus altered from the values intended, which are demonstrated below. After removing the supernatant, a proper amount of pepsin in 5 mM Tris*HCl/0.2 M NaCl (pH 8) buffer was added to each of the 8 samples with the volumes and concentrations specified below:
Reaction time: 4 hours 2. 0.00398 g of pepsin
Reaction time: 6 days
Reaction time: 4 hours 4. 0.00470 g of pepsin
Reaction time: 6 days
Reaction time: 4 hours 6. 0.00465 g of pepsin
Reaction time: 6 days
Reaction time: 4 hours 8. 0.00584 g of pepsin
Reaction time: 6 days
Approximately 2 hours into the reaction, no visible color change was observed. |